GTPF analysis

Integration of DropSeq and Cytometer. Left panel: a block-chart of the instrument. A cytometer (A1 or A2) supplies stream of cells (blue dots) with reference beads (red dots) to the droplet generator (C). Simultaneously, IBCs are supplied to the same droplet generator from a serial position-encoding chip, where IBC identification is performed by a serial number (B1) or from a pool of IBCs with unique IDs where identification is performed by a combination of fluorescent tags at the detector (B2). Reference beads (A1,2 red dots, B1,2 violet dots) are used for error managing. After pairing cells with IBCs (C), droplet compositions are monitored (D) and processed/sequenced as in regular Drop-Seq systems. Right panel: an on-chip instrument layout. Electroosmotic pumps (channels, separately accessed by electrodes and containing ionized groups on surface, Ai) are employed to propel and direct cells through detection spot and sorting junction (Aii). Cells of interest are equidistantly stored in cell accumulator (Aiii) and when supplied to droplet generator (Ci). Simultaneously, IBCs are supplied to the droplet generator from a chip (B1i) or from an external source (i.e. left panel B2). After pairing detector (Di), cells are processed/sequenced as in regular Drop-Seq systems