Antimicrobial Resistance Research Group

Contact information: 

Room 14, 23 Lenina st., Tyumen.
Mailing address: 6 Volodarskogo street, 625003, Tyumen.
Tel. +7(3452) 45-51-07
a.s.vasilchenko@utmn.ru

Areas of Research:

1. Study of the structural and functional characteristics of novel antimicrobial peptides of plant and microbiological origins. 
2. Elucidation of the sub-inhibitory effects of antimicrobial peptides. 
3. The development of antimicrobial peptide based bioconjugates capable of targeted action on bacterial cells. 
4. The development of a new generation of biofungicides. 

Research Team:

Alexey Vasilchenko, PhD in Microbiology


Scopus ID 36967720800
https://www.researchgate.net/profile/Alexey_Vasilchenko2

Areas of interests: 
1. Development of plant-protection strategies using antimicrobial peptides. 
2. Sub-inhibitory effects of antimicrobial peptides with regards to their structural and functional properties. 

Eugene Rogozhin, PhD in Bioorganic Chemistry

Scopus ID 6701467007
https://www.researchgate.net/profile/Eugene_Rogozhin

Areas of interests: 
Structural and functional analysis of antimicrobial peptides. 

Valeria Kalchugina, MSc Student, Plant Biosecurity Program

Scopus ID 57195522547 

Area of interest: 
Development of a new generation of antifungals with a basis in antimicrobial peptides. 

William Julian, MSc Student, Plant Biosecurity Program

Areas of interest: 
The development of antimicrobial peptide based bioconjugates capable of targeted action on bacterial cells. 

Current Projects:

1. RSF 18-74-10073 “Analysis of Antimicrobial Molecules of Plants and Fungi: A New Generation of Biofungicides”. 
2. RFBR 18-34-20058 “Comparative Analysis of Antimicrobial Peptides of Wild and Cultivated Origin and the Elucidation of the Molecular Mechanism of Action”.

Sample research topics for postdocs and PhD students:

1. Molecular mechanisms of microbial resistance and virulence. 
2. Application of antimicrobial peptides of various origins in plant protection. 

Selected Publications: 

1. Vasilchenko A.S., Nikiyan H.N., Deryabin D.G. Atomic force microscopy study of magainin 2 versus human platelet extract action on Escherichia coli and Bacillus cereus  // Journal of Biological Research. – 2013. – N 19 p. 3-9. (IF ISI JCR=1.36, Q2).

2.  Tatlybaeva E.B. Nikiyan H.N., Vasilchenko A.S., Deryabin D.G. Atomic force microscopy recognition of protein A on Staphylococcus aureus cell surfaces by labelling with IgG–Au conjugates //  Beilstein Journal of Nanotechnology. – 2013 - № 4. - P. 743–749. doi: 10.3762/bjnano.4.84 (IF ISI JCR=2.374, Q1).

3. Kamaeva A.A., Vasilchenko A.S., Deryabin D.G. Atomic Force Microscopy Reveals a Morphological Differentiation of Chromobacterium violaceum Cells Associated with Biofilm Development and Directed by N-Hexanoyl-L-Homoserine Lactone // PLoS ONE, 2014,9(8): e103741. doi:10.1371/journal.pone.0103741. (IF ISI JCR =3.53, Q1).

4. Vasilchenko A., Dymova V., Kartashova O., Sycheva M. Morphofunctional Reaction of Bacteria Treated with Antimicrobial Peptides Derived from Farm Animal Platelets // Probiotics Antimicrob Proteins – 2014, DOI: 10.1007/s12602-014-9172-4. (IF ISI JCR = 2.3, Q2).

5. Alexey S. Vasilchenko, Mikhail Yuryev, Dmitry Yu Ryazantsev, Sergey K. Zavriev, Alexey V. Feofanov, Eugene V. Grishin, Eugene A. Rogozhin. Studying of cellular interaction of hairpin-like peptide EcAMP1 from barnyard grass (Echinochloa crusgalli L.) seeds with plant pathogenic fungus Fusarium solani using microscopy techniques // Scanning – 2016,  38(6):591-598  (IF ISI JCR =1.5, Q3).

4. Alexey S. Vasilchenko, Alexey N. Smirnov, Sergei K. Zavriev, Eugene V. Grishin, Anastasia V. Vasilchenko, Eugene A. Rogozhin. Novel Thionins from Black Seed (Nigella sativa L.) Demonstrate Antimicrobial Activity // Int J Pept Res Ther – 2017,  23(2):171–180, DOI 10.1007/s10989-016-9549-1. (IF ISI JCR = 1.13, Q3).

5. Alexey S. Vasilchenko, Eugene A. Rogozhin, Alexander V. Valyshev. Purification of a novel bacteriocin-like inhibitory substance produced by Enterococcus faecium ICIS 8 and characterization of its mode of action // Microbial Drug Resistance – 2017, 23(4) p.447-456, DOI: 10.1089/mdr.2016.0069 (IF ISI JCR = 2.59, Q1).

6. Vasilchenko A.S., Rogozhin E.A., Vasilchenko A.V., Kartashova O.L., Sycheva M.V. Novel hemoglobin-derived antimicrobial peptides from chicken (Gallus gallus) blood: purification, structural aspects, and biological activity // Journal of Applied Microbiology – 2016, 121(6):546-1557.1  DOI: 10.1111/jam.13286 (IF ISI JCR = 2.17, Q2).

7.Vasilchenko A. S., Vasilchenko A.V., Pashkova T.M., Smirnova M.P.,  Kolodkin N. I., Manukhov I.V., Zavilgelsky G.B., Sizova E.A., Kartashova O.L., Simbirtsev A. S., Rogozhin E.A., Duskaev G.K.,  Sycheva M.V. Antimicrobial activity of the indolicidin-derived novel synthetic peptide In-58 // Journal of Peptide Science – 2017, 23(12):855-863 (ИФ ISI JCR = 1.97, Q2).

8. Vasilchenko A.S., Vasilchenko A.V., Valyshev A. V., Rogozhin A.E. A Novel High-Molecular-Mass Bacteriocin Produced by Enterococcus faecium: Biochemical Features and Mode of Action // Probiotics Antimicrob Proteins  -  2018, 10:427–434. https://doi.org/10.1007/s12602-018-9392-0. (IF ISI JCR = 2.4, Q2). 

9. Pashkova T.M., Vasilchenko A.S., Khlopko Y.A., Kochkina E.E., Kartashova O.L., Sycheva M.V. Genome Sequence of Enterococcus faecium Strain ICIS 96 Demonstrating Intermicrobial Antagonism Associated with Bacteriocin Production // Genome Announc. 2018 Mar 8;6(10). pii: e00126-18. doi: 10.1128/genomeA.00126-18.

10. Vasilchenko AS, Gritsenko VA, Kosyan DB, Rogozhin EA. A Low-Molecular-Weight Compound Derived from Human Leukocytes Determines a Bactericidal Activity of the Interferon Preparation // Probiotics Antimicrob Proteins. 2018 Sep 13. doi: 10.1007/s12602-018-9463-2. (IF ISI JCR = 2.4, Q2).
11. Rogozhin E.A., Sadykova V.S., Baranova A.A., Vasilchenko A.S., Lushpa V.A., MineevK.S., Georgieva M.L., Kul’ko A.B., Krasheninnikov M.E., Lyundup A.V., Vasilchenko A.V., Andreev Y.A. A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis Alkalina // Molecules. – 2018 (accepted) (IF ISI JCR =3.02, Q1)

Media Coverage of the Lab Research:

https://www.utmn.ru/presse/ekspertnoe-mnenie/527677/ (Russian source)
https://tass.ru/ural-news/5065888 (Russian source)
https://ria.ru/science/20180123/1513098816.html (Russian source)